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Course

Microbiology of Activated Sludge

Attributes	CEUs: 1.5 FBPE PDHs 0004040: 15 FDEP OCP WW CEUs: 1.5: Intermediate: 4191 Time Day One: 8:00 a.m. - 4:30 p.m. Time Day Two: 8:00 a.m. - 4:30 p.m.
Coordinator	Stephanie West, (352) 392-9570 x216
Description	This interactive two-day course includes hands-on use of advanced microscopes, slide preparation and filament staining techniques. Students will learn proper care and use of microscopes as well as activated sludge process testing and troubleshooting. The course focuses on the valuable information a microscope provides for controlling and troubleshooting an activated sludge wastewater treatment plant. Topics covered include: process control tests interpreting the results calculating loading and waste sludge rates applying the data This is an excellent course for intermediate to advanced level operators. Students are invited to bring small MLSS samples from their facility to use during slide preparation and organism identification exercises. Students will receive the AWWA book "Wastewater Microbiology A Handbook for Operators" a $89 value and also troubleshooting charts geared toward solving commonly encountered problems at an activated sludge treatment plant What our students are saying about this course: "I don’t know what else to give this course other than a excellent +. I must admit I mostly was interested in getting my CEU’s, however; this is the only 2-day course I have taken for CEU’s that I wish I could stay longer.” “Recommended this class to all WW operators.” "The most valuable portion of the course was the hands-on lab work" Course Instructor: Ron Trygar, CET Ron Trygar, CET-Ron has recently joined the UF TREEO Center staff as our Senior Training Specialist for Water and Wastewater. He has worked in the wastewater industry for twenty five years in a variety of locations and jobs. In 1998 Ron became a Certified Environmental Trainer (CET) and has since provided training for associations and regulatory agencies such as FDEP; UF-TREEO Center; FW&PCOA Short Schools; USABlueBook; FWEA sponsored training events and local school environmental programs. He has also assisted the U.S. EPA in providing flow measurement training in Tallahassee, and along with the FDEP Northeast District, he helped begin the FRWA/FDEP joint operator certification review classes that are still given around the state today. Ron holds a Florida class A wastewater treatment operator’s license and a Florida class C drinking water operator’s license.

Attributes

CEUs: 1.5
FBPE PDHs 0004040: 15
FDEP OCP WW CEUs: 1.5: Intermediate: 4191
Time Day One: 8:00 a.m. - 4:30 p.m.
Time Day Two: 8:00 a.m. - 4:30 p.m.

Coordinator

Stephanie West, (352) 392-9570 x216

Description

This interactive two-day course includes hands-on use of advanced microscopes, slide preparation and filament staining techniques. Students will learn proper care and use of microscopes as well as activated sludge process testing and troubleshooting. The course focuses on the valuable information a microscope provides for controlling and troubleshooting an activated sludge wastewater treatment plant.

Topics covered include:

process control tests
interpreting the results
calculating loading and waste sludge rates
applying the data

This is an excellent course for intermediate to advanced level operators. Students are invited to bring small MLSS samples from their facility to use during slide preparation and organism identification exercises.

Students will receive the AWWA book "Wastewater Microbiology A Handbook for Operators" a $89 value and also troubleshooting charts geared toward solving commonly encountered problems at an activated sludge treatment plant

What our students are saying about this course:

"I don’t know what else to give this course other than a excellent +. I must admit I mostly was interested in getting my CEU’s, however; this is the only 2-day course I have taken for CEU’s that I wish I could stay longer.”

“Recommended this class to all WW operators.”

"The most valuable portion of the course was the hands-on lab work"

Course Instructor: Ron Trygar, CET

Ron Trygar, CET-Ron has recently joined the UF TREEO Center staff as our Senior Training Specialist for Water and Wastewater. He has worked in the wastewater industry for twenty five years in a variety of locations and jobs. In 1998 Ron became a Certified Environmental Trainer (CET) and has since provided training for associations and regulatory agencies such as FDEP; UF-TREEO Center; FW&PCOA Short Schools; USABlueBook; FWEA sponsored training events and local school environmental programs. He has also assisted the U.S. EPA in providing flow measurement training in Tallahassee, and along with the FDEP Northeast District, he helped begin the FRWA/FDEP joint operator certification review classes that are still given around the state today. Ron holds a Florida class A wastewater treatment operator’s license and a Florida class C drinking water operator’s license.

Microbiology of Activated Sludge

Sept 3-4, 2008

Agenda

Day 1

8:00am-8:30 Welcome & Introductions

8:30-9:45 Wastewater Microscopy

Types of Microscopes

Proper Use and Maintenance of Microscopes

Which Microscope Do I Need?

9:45-10:00 Break

10:00-12:00 Wastewater Microscopy, (cont’d)

Sample Collection

Wet Mount Slide Preparation & Demonstration

Live Organism Identification Using Video Camera

What to Look For

12:00-1:00 Lunch

1:00-2:45 Hands-on Use of Microscopes

Prepare Wet Mount Slides

View using Brightfield Microscopy, and Phase-contrast Microscopy

2:45-3:00 Break

3:00-4:30 Using The Microscope as a Process Control Tool

What Did you See?

What Does it Mean?

How Does It Relate to Plant Operation?

Preparing Smears for Next Day Use

4:30 Adjourn

Day 2

8:00-10:00 Activated Sludge Troubleshooting

Settleability and Diluted Settleability, SVI

Identifying Bulking Conditions

10:00-10:15 Break

10:15-12:00 Identifying Filaments

Demonstration of Staining Procedures

Gram, Niessor, PHB Stains, India Ink Reverse Staining, Sheath Staining

12:00-1:00 Lunch

1:00-2:45 Hands on Microscope Use

Perform Staining

Identify Filaments Using Reference Books and Charts

3:00-4:30 Troubleshooting the Activated Sludge Process

Analyzing the Data

Using Troubleshooting Charts

When to Take Corrective Action

Which Corrective Action is Best?

4:30 Adjourn